Measuring enzyme activities under standardized in vivo-like conditions for systems biology
نویسندگان
چکیده
منابع مشابه
Standardized assay medium to measure Lactococcus lactis enzyme activities while mimicking intracellular conditions.
Knowledge of how the activity of enzymes is affected under in vivo conditions is essential for analyzing their regulation and constructing models that yield an integrated understanding of cell behavior. Current kinetic parameters for Lactococcus lactis are scattered through different studies and performed under different assay conditions. Furthermore, assay conditions often diverge from conditi...
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ژورنال
عنوان ژورنال: FEBS Journal
سال: 2010
ISSN: 1742-464X
DOI: 10.1111/j.1742-4658.2009.07524.x